It was next seen in Aliens vs. Predator: Requiem. It attacked a pair of Predators on the Predator scout ship. The ensuing battle caused the hull to rupture and the ship crashed in Gunnison, Colorado. The Predalien and multiple facehuggers escaped. They went into the sewers where they impregnated homeless people in order to create drones. The Predalien managed to insert an embryo straight into a woman's throat in order for it to gestate.
Wolf responded to the distress call of the scout ship.
Before long, the Predalien and the loyal alien drones were taking over the town. They began to build a resin nest in the hospital.
As Wolf and the Predalien battled, the town was destroyed by a nuclear blast to eradicate all the alien lifeforms.