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Dr. Charles Ashford, played by Jared Harris, is a character in the film Resident Evil: Apocalypse.

A high-ranking scientist with Umbrella, Ashford is evacuated out of Raccoon City when word of The Hive being reopened and the infected within escaping forces Umbrella to pull all their employees in Raccoon City out of the area before establishing their quarantine perimeter. Although Ashford is safely evacuated, his daughter Angela, who was pulled out of her class at Raccoon City Elementary, doesn't make the rendezvous when her evacuation vehicle is struck by a tanker truck driven by a T-Virus infected driver, killing the Umbrella security personnel sent to retrieve her, while she escapes and retreats back to her school, remaining there as the virus infects her teachers, classmates, and other residents of the city, including a set of R.P.D. K-9 police dogs when the school was properly evacuated by the R.P.D. after suggestion from former S.T.A.R.S. officer Jill Valentine.

Fearing for his daughter's safety, Ashford is able to hack into Raccoon City's CCTV system, and contacting Alice and other survivors, such as Jill, Lloyd Jefferson Wade, and members of the U.B.C.S., like Carlos Oliveira, he offers them a way out of the city before Umbrella has it nuked to cover up their actions in return for the rescue and return of his daughter. Though Angela is safely recovered, Ashford's actions are discovered by Major Timothy Cain, and he is taken hostage as Cain confronts the escapees at the rendezvous point at Raccoon City Hall. When Alice refuses to fight Nemesis under Cain's orders, Ashford is mercilessly executed by Cain as a show of force to get her to fight Nemesis, with Cain threatening to kill Angela, Jill, Carlos, and L.J. next if she continues to resist.

After Cain is left to fend for himself by Alice while she and the other survivors escape before the city is nuked, Ashford gets his revenge for Cain killing him when the T-virus reanimates him as a zombie, and he is the first to reach Cain and begins to devour him before the rest of the horde join him. They are all vaporized when the nuke detonates over Raccoon City Hall.

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