Alexander Ashford is the late-patriarch of the Ashford family. The son of the late-Edward Ashford, Alexander set out to restore their family's legacy by creating twins Alfred and Alexia Ashford from genetic material of his legendary ancestor Veronica.
He also develops the Linear Launcher to deal Alexia after growing concerned of her growing superiority complex. After the twins learn of their true origins, they infect Alexander with the T-Veronica virus, transforming him into a creature referred to as Nosferatu. The twins then imprison him deep within an Arctic facility, where he remains for the following years.
Alexander breaks free during the events of Resident Evil Code: Veronica, where he confronts Claire Redfield and Steve Burnside as they attempt to flee the facility. Alexander is finally killed when Redfield shoots out his heart. His body is later recovered by her brother Chris Redfield to obtain his pendant to access the depths of the facility. Alexander retains the Nosferatu role in game's retelling in Resident Evil: The Darkside Chronicles. However, he appears in a message recording as part of the Arctic facility's self-destruct sequence.